Achieve High-Density Cell Culture With A Stirred-Tank Bioreactor

By James Jarvis

This application report presents a simple protocol for achieving high-density culture of Spodoptera frugiperda (Sf9) cells using a New Brunswick bench-top, autoclavable stirred-tank reactor with a spin-filter assembly. Factors such as substrate concentration and metabolite buildup can be limiting for culture growth and viability at high densities. Using the spin filter in a 3.0 L vessel (2.0 L working volume) attached to the BioFlo®/CelliGen® 115 cabinet, a cell density of 18.24 x 106 cells with viability over 90 % was achieved, outperforming the batch or fedbatch process.